Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.

ABSTRACT: Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood. While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP). Previous studies targeted MGPs isolated by filtration. We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater. Seawater was

SUBMITTER: Al-Wahaibi ASM 

PROVIDER: S-EPMC10048003 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature