Validation of Probabilistic Genotyping Software for Single Cell STR Analysis.

ABSTRACT: Probabilistic genotyping (PG) and its associated software has greatly aided in forensic DNA mixture analysis, with it primarily being applied to mixed DNA profiles obtained from bulk cellular extracts. However, these software applications do not always result in probative information about the identity of all donors to said mixtures/extracts. This is primarily due to mixture complexity caused by overlapping alleles and the presence of artifacts and minor donors. One way of reducing mixture complexity is to perform direct single cell subsampling of the bulk mixture prior to genotyping and interpretation. The analysis of low template DNA samples, including from single or few cells, has also benefited from the application of PG methods. With the application of PG, multiple cell subsamples ori

SUBMITTER: Huffman K 

PROVIDER: S-EPMC10048617 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature