An Insight into the Essential Role of Carbohydrate-Binding Modules in Enzymolysis of Xanthan.

ABSTRACT: To date, due to the low accessibility of enzymes to xanthan substrates, the enzymolysis of xanthan remains deficient, which hinders the industrial production of functional oligoxanthan. To enhance the enzymatic affinity against xanthan, the essential role of two carbohydrate binding modules-MiCBMx and PspCBM84, respectively, derived from Microbacterium sp. XT11 and Paenibacillus sp. 62047-in catalytic properties of endotype xanthanase MiXen were investigated for the first time. Basic characterizations and kinetic parameters of different recombinants revealed that, compared with MiCBMx, PspCBM84 dramatically increased the thermostability of endotype xanthanase, and endowed the enzyme with higher substrate affinity and catalytic efficiency. No

SUBMITTER: Ni X 

PROVIDER: S-EPMC10049358 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature