Amniotic Mesenchymal-Derived Extracellular Vesicles and Their Role in the Prevention of Persistent Post-Breeding Induced Endometritis.

ABSTRACT: Persistent post-breeding induced endometritis (PPBIE) is considered a major cause of subfertility in mares. It consists of persistent or delayed uterine inflammation in susceptible mares. There are many options for the treatment of PPBIE, but in this study, a novel approach aimed at preventing the onset of PPBIE was investigated. Stallion semen was supplemented with extracellular vesicles derived from amniotic mesenchymal stromal cells (AMSC-EVs) at the time of insemination to prevent or limit the development of PPBIE. Before use in mares, a dose-response curve was produced to evaluate the effect of AMSC-EVs on spermatozoa, and an optimal concentration of 400 × 10⁶ EVs with 10 × 10⁶ spermatozoa/mL was identified. At this concentration, sperm mobility parameters were n

SUBMITTER: Lange-Consiglio A 

PROVIDER: S-EPMC10049450 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature