Global Analysis of Dark- and Heat-Regulated Alternative Splicing in Arabidopsis.

ABSTRACT: Alternative splicing (AS) is one of the major post-transcriptional regulation mechanisms that contributes to plant responses to various environmental perturbations. Darkness and heat are two common abiotic factors affecting plant growth, yet the involvement and regulation of AS in the plant responses to these signals remain insufficiently examined. In this study, we subjected Arabidopsis seedlings to 6 h of darkness or heat stress and analyzed their transcriptome through short-read RNA sequencing. We revealed that both treatments altered the transcription and AS of a subset of genes yet with different mechanisms. Dark-regulated AS events were found enriched in photosynthesis and light signaling pathways, while heat-regulated AS events were enriched in responses to abiotic stresses but not

SUBMITTER: Zhang D 

PROVIDER: S-EPMC10049525 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature