Distinguishing cell-cell complexes from dual lineage cells using single-cell transcriptomics is not trivial.

ABSTRACT: In their recent correspondence, Jie et al. strongly defend that the DE cell population they discovered are always dual lineage co-expressing cells and not complexes of B cells and T cells, which we have previously described as frequently present in single-cell RNA sequencing data. Here, we respond to the specific arguments made in their correspondence. Specifically, we demonstrate that the presence of a gene signature in a given cell population is not enough to ascertain that it does not contain cell-cell complexes, or that it represents a biologically distinct cell type. We also show that the gene signature of DE cells contains several genes from the myeloid lineage, suggesting either that their DE cells are a triple-lineage co-expressing cell, or a three-component cell aggregate. Finally

SUBMITTER: Burel JG 

PROVIDER: S-EPMC10049842 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature