Comparative phyloproteomics identifies conserved plasmodesmal proteins.

ABSTRACT: Plasmodesmata are cytosolic bridges, lined by the plasma membrane and traversed by endoplasmic reticulum; plasmodesmata connect cells and tissues, and are critical for many aspects of plant biology. While plasmodesmata are notoriously difficult to extract, tissue fractionation and proteomic analyses can yield valuable knowledge of their composition. Here we have generated two novel proteomes to expand tissue and taxonomic representation of plasmodesmata: one from mature Arabidopsis leaves and one from the moss Physcomitrium patens, and leveraged these and existing data to perform a comparative analysis to identify evolutionarily conserved protein families that are associated with plasmodesmata. Thus, we identified β-1,3-glucanases, C2 lipid-binding proteins, and tetraspanins as core plasmo

SUBMITTER: Johnston MG 

PROVIDER: S-EPMC10049917 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature