Comparing ¹³C methyl and deuterated methyl isotopic labeling for the quantification of methyl cellulose patterns using mass spectrometry.

ABSTRACT: The methyl substitution along and among the polymer chains of methyl cellulose (MC) is commonly analyzed by ESI-MS after perdeuteromethylation of the free-OH groups and partial hydrolysis to cello-oligosaccharides (COS). This method requires a correct quantification of the molar ratios of the constituents belonging to a particular degree of polymerization (DP). However, isotopic effects are most pronounced for H/D since their mass difference is 100%. Therefore, we investigated whether more precise and accurate results could be obtained for the methyl distribution of MC by MS of ¹³CH₃ instead of CD₃-etherified O-Me-COS. Internal isotope labeling with ¹³CH₃ makes the COS of each DP chemically and physically much more similar, reducing ma

SUBMITTER: Schleicher S 

PROVIDER: S-EPMC10050035 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature