Optogenetic Spreading Depolarizations Do Not Worsen Acute Ischemic Stroke Outcome.

ABSTRACT:

Background

Spreading depolarizations (SDs) are believed to contribute to injury progression and worsen outcomes in focal cerebral ischemia because exogenously induced SDs have been associated with enlarged infarct volumes. However, previous studies used highly invasive methods to trigger SDs that can directly cause tissue injury (eg, topical KCl) and confound the interpretation. Here, we tested whether SDs indeed enlarge infarcts when induced via a novel, noninjurious method using optogenetics.

Methods

Using transgenic mice expressing channelrhodopsin-2 in neurons (Thy1-ChR2-YFP), we induced 8 optogenetic SDs to trigger SDs noninvasively at a remote cortical location in a noninjurious manner during 1-hour distal microvascular clip or proximal an endovascular filament occlusi

SUBMITTER: Sugimoto K 

PROVIDER: S-EPMC10050120 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature