Sperm quality metrics were improved by a biomimetic microfluidic selection platform compared to swim-up methods.

ABSTRACT: Sperm selection is an essential component of all assisted reproductive treatments (ARTs) and is by far the most neglected step in the ART workflow in regard to technological innovation. Conventional sperm selection methodologies typically produce a higher total number of sperm with variable motilities, morphologies, and levels of DNA integrity. Gold-standard techniques, including density gradient centrifugation (DGC) and swim-up (SU), have been shown to induce DNA fragmentation through introducing reactive oxygen species (ROS) during centrifugation. Here, we demonstrate a 3D printed, biologically inspired microfluidic sperm selection device (MSSP) that utilizes multiple methods to simulate a sperms journey toward selection. Sperm are first selected based on their motility and boundary-foll

SUBMITTER: Vasilescu SA 

PROVIDER: S-EPMC10050147 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature