Transcriptome analysis of newly established carboplatin-resistant ovarian cancer cell model reveals genes shared by drug resistance and drug-induced EMT.

ABSTRACT:

Background

In ovarian cancer (OC) therapy, even initially responsive patients develop drug resistance.

Methods

Here, we present an OC cell model composed of variants with differing degrees of acquired resistance to carboplatin (CBP), cross-resistance to paclitaxel, and CBP-induced metastatic properties (migration and invasion). Transcriptome data were analysed by two approaches identifying differentially expressed genes and CBP sensitivity-correlating genes. The impact of selected genes and signalling pathways on drug resistance and metastatic potential, along with their clinical relevance, was examined by in vitro and in silico approaches.

Results

TMEM200A and PRKAR1B were recognised as potentially involved in both phenomena, also having high predictive and prognosti

SUBMITTER: Kralj J 

PROVIDER: S-EPMC10050213 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature