Transcriptomics of CD29⁺/CD44⁺ cells isolated from hPSC retinal organoids reveals a single cell population with retinal progenitor and Muller glia characteristics.

ABSTRACT: Müller glia play very important and diverse roles in retinal homeostasis and disease. Although much is known of the physiological and morphological properties of mammalian Müller glia, there is still the need to further understand the profile of these cells during human retinal development. Using human embryonic stem cell-derived retinal organoids, we investigated the transcriptomic profiles of CD29⁺/CD44⁺ cells isolated from early and late stages of organoid development. Data showed that these cells express classic markers of retinal progenitors and Müller glia, including NFIX, RAX, PAX6, VSX2, HES1, WNT2B, SOX, NR2F1/2, ASCL1 and VIM, as early as days 10-20 after initiation of retinal differentiation. Expression of genes upregulated in CD29⁺/CD44⁺

SUBMITTER: Eastlake K 

PROVIDER: S-EPMC10050419 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature