Human umbilical cord blood cells suffer major modification by fixatives and anticoagulants.

ABSTRACT: Introduction: Developing techniques for the tagless isolation of homogeneous cell populations in physiological-like conditions is of great interest in medical research. A particular case is Gravitational Field-Flow Fractionation (GrFFF), which can be run avoiding cell fixation, and that was already used to separate viable cells. Cell dimensions have a key role in this process. However, their dimensions under physiological-like conditions are not easily known since the most diffused measurement techniques are performed on fixed cells, and the fixation used to preserve tissues can alter the cell size. This work aims to obtain and compare cell size data under physiological-like conditions and in the presence of a fixative. Methods: We developed a new protocol that allows the ana

SUBMITTER: Danusso R 

PROVIDER: S-EPMC10050555 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature