Characterization of complement C3 as a marker of alpha-amanitin toxicity by comparative secretome profiling.

ABSTRACT: In the human body, proteins secreted into peripheral blood vessels are known as the secretome, and they represent the physiological or pathological status of cells. The unique response of cells to toxin exposure can be confirmed via secretome analysis, which can be used to discover toxic mechanisms or exposure markers. Alpha-amanitin (α-AMA) is the most widely studied amatoxin and inhibits transcription and protein synthesis by directly interacting with RNA polymerase II. However, secretory proteins released during hepatic failure caused by α-AMA have not been fully characterized. In this study, we analyzed the secretome of α-AMA-treated Huh-7 cells and mice using a comparative proteomics technique. Overall, 1440 and 208 proteins were quantified in cell media and mouse serum, respec

SUBMITTER: Kim D 

PROVIDER: S-EPMC10050625 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature