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Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application.

ABSTRACT: One of the groups of organic compounds with potential use in medicine and pharmacy is phthalimide derivatives. They are characterized by a wide range of properties such as antibacterial, antifungal, and anti-inflammatory. In this study, we focused on research on four phthalimide derivatives with proven non-toxicity, which are cyclooxygenase inhibitors. With the use of molecular docking study and spectroscopic methods, such as fluorescence, circular dichroism, and FT-IR spectroscopies, we analyzed the way the tested compounds interact with plasma proteins. Among the many proteins present in the plasma, we selected three: albumin, α1-acid glycoprotein, and gamma globulin, which play significant roles in the human body. The obtained results showed that all tested compounds bind to the analyzed proteins. They interact most strongly with albumin, which is a transport protein. However, interactions with serum albumin and orosomucoid do not cause significant changes in their structures. Only in the case of gamma globulins significant changes were observed in protein secondary structure.

SUBMITTER: Krzyzak E

PROVIDER: S-EPMC10051393 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application.

Krzyżak Edward E Kotynia Aleksandra A Szkatuła Dominika D Marciniak Aleksandra A

Life (Basel, Switzerland) 20230310 3

One of the groups of organic compounds with potential use in medicine and pharmacy is phthalimide derivatives. They are characterized by a wide range of properties such as antibacterial, antifungal, and anti-inflammatory. In this study, we focused on research on four phthalimide derivatives with proven non-toxicity, which are cyclooxygenase inhibitors. With the use of molecular docking study and spectroscopic methods, such as fluorescence, circular dichroism, and FT-IR spectroscopies, we analyze ...[more]

PMID: 36983915

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Project description:Ultrasound-assisted synthesis of water soluble poly(o-phenylenediamine) (POPD) and its doping with Acid Orange (AO), Fluorescein (Fluo) and Rhodamine-6G (R6G) dyes was carried out with a view to enhance the photophysical properties of POPD. XPS studies confirmed that doping of POPD occured through hydrogen bonding between NH group of POPD and C=O/SO-, S=O groups of the dyes. The presence of strong hydrogen bonding was also confirmed via UV-vis studies by the addition of urea and sodium chloride to the dye modified POPD adducts. Molar extinction coefficient of these adducts was found to bear a close relationship with the molecular structure. Fluorescence life time, (τf,) was found to be lowest (1.8 ns) for AO-POPD and highest (3.2 ns) for Fluo-POPD. The structure of AO-POPD was more strained, while that of Fluo-POPD was least strained. Intrinsic fluorescence decay constant, (k0f) showed increasing values for POPD, AO-POPD, Fluo-POPD, R6G-POPD as 0.071, 0.072, 0.153, and 0.172 (108 s-1), which could be correlated to the increasing strain-free molecular structure of the adducts. Circular dichroism spectra (CD) of BSA in presence of POPD and R6G- POPD revealed that it partially broke its helical structure, while Fluo-POPD and AO-POPD showed enhancement in the helical content. The 3-D fluorescence studies confirmed enhancement in hydrophobicity of POPD and R6G- POPD and increase in hydrophylicity of AO-POP and Fluo-POPD in the microenvironment of tryptophan residue-213 of BSA. Fluo-POPD and R6G-POPD adducts were chosen to find out the lowest detection limit (LOD) of BSA by differential pulse voltammetry (DPV) which was found to be 1.35 nM, and 1.65 nM using Fluo-POPD and R6G -POPD respectively. The binding constant of BSA with Fluo-POPD- and R6G-POPD was obtained as 3.98 × 106 Lmol-1 and 5.27 × 102 Lmol-1. These polymers could therefore, be used for the detection of BSA. Live cell imaging revealed that POPD nanoparticles were bound to the outer membrane of E. coli, while R6G-POPD, showed penetration into the cytoplasm and excellent labeling of E. coli. This facile technique could be used to design tunable biomarkers by tailoring the conjugated polymer with a desired dye molecule.

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Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application.

Publications

Spectroscopic and Theoretical Analysis of the Interaction between Plasma Proteins and Phthalimide Analogs with Potential Medical Application.

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