Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer.

ABSTRACT: The cowpea chlorotic mottle virus (CCMV) is a plant virus explored as a nanotechnological platform. The robust self-assembly mechanism of its capsid protein allows for drug encapsulation and targeted delivery. Additionally, the capsid nanoparticle can be used as a programmable platform to display different molecular moieties. In view of future applications, efficient production and purification of plant viruses are key steps. In established protocols, the need for ultracentrifugation is a significant limitation due to cost, difficult scalability, and safety issues. In addition, the purity of the final virus isolate often remains unclear. Here, an advanced protocol for the purification of the CCMV from infected plant tissue was developed, focusing on efficiency, economy, and final purity. T

SUBMITTER: Tscheuschner G 

PROVIDER: S-EPMC10051510 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature