Cortical Astrocyte Progenitors and Astrocytes from Human Pluripotent Stem Cells.

ABSTRACT: Astrocytes coordinate several homeostatic processes of the central nervous system and play essential roles for normal brain development and response to disease conditions. Protocols for the conversion of human induced pluripotent stem cells (hiPSCs) into mature astrocytes have opened to the generation of in vitro systems to explore astrocytes' functions in living human cell contexts and patient-specific settings. In this study, we present an optimized monolayer procedure to commit hiPSC-derived cortical progenitors into enriched populations of cortical astrocyte progenitor cells (CX APCs) that can be further amplified and efficiently differentiated into mature astrocytes. Our optimized system provides a valid tool to explore the role of these cells in neurodevelopmental and neuropsy

SUBMITTER: Battistella I 

PROVIDER: S-EPMC10051695 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature