Recent advances in the delivery and applications of nonviral CRISPR/Cas9 gene editing.

ABSTRACT: The CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 genome editing system has been a major technological breakthrough that has brought revolutionary changes to genome editing for therapeutic and diagnostic purposes and precision medicine. With the advent of the CRISPR/Cas9 system, one of the critical limiting factors has been the safe and efficient delivery of this system to cells or tissues of interest. Several approaches have been investigated to find delivery systems that can attain tissue-targeted delivery, lowering the chances of off-target editing. While viral vectors have shown promise for in vitro, in vivo and ex vivo delivery of CRISPR/Cas9, their further clinical applications have been restricted due to shortcomings including limited cargo packaging capaci

SUBMITTER: Sinclair F 

PROVIDER: S-EPMC10052255 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature