Second-Harmonic Generated Quantifiable Fibrosis Parameters Provide Signatures for Disease Progression and Regression in Nonalcoholic Fatty Liver Disease.

ABSTRACT:

Introduction

The current ordinal fibrosis staging system for nonalcoholic steatohepatitis (NASH) has a limited dynamic range. The goal of this study was to determine if second-harmonic generated (SHG) quantifiable collagen fibrillar properties (qFP) and their derived qFibrosis score capture changes in disease progression and regression in a murine model of NASH, in which disease progression can be induced by a high fat sugar water (HFSW) diet and regression by reversal to chow diet (CD).

Methods

DIAMOND mice were fed a CD or HFSW diet for 40 to 52 weeks. Regression related changes were studied in mice with diet reversal for 4 weeks after 48 to 60 weeks of a HFSW diet.

Results

As expected, mice on HFSW developed steatohepatitis with stage 2 to 3 fibrosis between weeks

SUBMITTER: Ng N 

PROVIDER: S-EPMC10052491 | biostudies-literature | 2023 Jan-Dec

REPOSITORIES: biostudies-literature