Quantitative analysis of biosurfactants in water samples by a modified oil spreading technique.

ABSTRACT: The oil spreading technique relies on biosurfactant to reduce the surface tension of an oil film and form an oil spreading ring in the center, and then judges the content of biosurfactant according to the diameter of the spreading ring. However, the instability and large errors of the traditional oil spreading technique limit its further application. In this paper, we modified the traditional oil spreading technique by optimizing the oily material, image acquisition and calculation method, which improves the accuracy and stability of the quantification of biosurfactant. We screened lipopeptides and glycolipid biosurfactants for rapid and quantitative analysis of biosurfactant concentrations. By selecting areas by color done by the software to modify image acquisition, the results showed th

SUBMITTER: Li H 

PROVIDER: S-EPMC10052697 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature