Discovery and characterization of the covalent SARS-CoV-2 3CL^pro inhibitors from Ginkgo biloba extract via integrating chemoproteomic and biochemical approaches.

ABSTRACT:

Background

The 3C-like proteases (3CL^pros) are cysteine-rich homodimeric proteins and can be covalently modified by numerous natural and synthetic compounds, which in turn, block the proteolytic activity or the formation of enzymatically active dimeric forms. Although herbal medicines have been widely used to treat COVID-19, identification of the key herbal constituents that can covalently modify the 3CL^pros in β-coronaviruses (CoVs) remains a big challenge.

Aims

To construct a comprehensive approach for efficient discovering the covalent SARS-CoV-2 3CL^pro inhibitors from herbal medicines. To decipher the key anti-SARS-CoV-2 3CL^pro constituents in Ginkgo biloba extract 50 (GBE50) and to study their anti-SARS-CoV-2 3CL^pro

SUBMITTER: Zhang YN 

PROVIDER: S-EPMC10052880 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature