Matrix Effects in GC-MS Profiling of Common Metabolites after Trimethylsilyl Derivatization.

ABSTRACT: Metabolite profiling using gas chromatography coupled to mass spectrometry (GC-MS) is one of the most frequently applied and standardized methods in research projects using metabolomics to analyze complex samples. However, more than 20 years after the introduction of non-targeted approaches using GC-MS, there are still unsolved challenges to accurate quantification in such investigations. One particularly difficult aspect in this respect is the occurrence of sample-dependent matrix effects. In this project, we used model compound mixtures of different compositions to simplify the study of the complex interactions between common constituents of biological samples in more detail and subjected those to a frequently applied derivatization protocol for GC-MS analysis, namely trimethylsilylation

SUBMITTER: Tarakhovskaya E 

PROVIDER: S-EPMC10053008 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature