Automated production of [¹¹C]butyrate for keto body PET imaging.

ABSTRACT: The report describes an updated, fully automated method for the production of [¹¹C]butyrate, validated for use in clinical studies. A commercially available GE Tracerlab FX_M synthesis module was reconfigured to allow for air-free introduction of n-propyl magnesium chloride and to incorporate Sep-Pak cartridges to simplify and shorten the purification process, as compared to purifying the product using traditional HPLC. The method takes 20 min from end-of-bombardment and reliably produces injectable doses of [¹¹C]butyrate (8029 ± 1628 MBq (217 ± 44 mCi), 14 % radiochemical yield based on [¹¹C]CO_2, non-decay corrected) in high radiochemical purity (>97 %), n = 3. With radiotracer in hand, PET scans of rats confirmed uptake of the radioph

SUBMITTER: Pakula RJ 

PROVIDER: S-EPMC10053181 | biostudies-literature | 2023 Jan-Feb

REPOSITORIES: biostudies-literature