Electrochemical Biosensor for Detection of the CYP2C19*2 Allele Based on Exonuclease Ⅲ.

ABSTRACT: Currently, the therapeutic effect of clopidogrel differs considerably among individuals and is thought to be closely related to the genetic polymorphism of CYP2C19. The CYP2C19*2 gene can reduce the antiplatelet aggregation effect of clopidogrel, which increases the risk of major cardiovascular adverse events in patients. In this research, we report a new type of biosensor for the highly sensitive detection of the CYP2C19*2 gene based on exonuclease III assisted electric signal amplification and the use of calixarene to enrich electrical signal substances. Specifically, under the best conditions, the logarithmic concentrations of the analytes have a good linear relationship with the peak current in the range of 0.01 fM to 100 pM and the detection limit is 13.49 aM. The

SUBMITTER: Chen S 

PROVIDER: S-EPMC10053321 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature