Screening for Primordial RNA-Peptide Interactions Using High-Density Peptide Arrays.

ABSTRACT: RNA-peptide interactions are an important factor in the origin of the modern mechanism of translation and the genetic code. Despite great progress in the bioinformatics of RNA-peptide interactions due to the rapid growth in the number of known RNA-protein complexes, there is no comprehensive experimental method to take into account the influence of individual amino acids on non-covalent RNA-peptide bonds. First, we designed the combinatorial libraries of primordial peptides according to the combinatorial fusion rules based on Watson-Crick mutations. Next, we used high-density peptide arrays to investigate the interaction of primordial peptides with their cognate homo-oligonucleotides. We calculated the interaction scores of individual peptide fragments and evaluated the influence of the pe

SUBMITTER: Jenne F 

PROVIDER: S-EPMC10053474 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature