Mediator Subunit Med15 Regulates Cell Morphology and Mating in Candida lusitaniae.

ABSTRACT: Candida lusitaniae is an emerging opportunistic pathogenic yeast capable of shifting from yeast to pseudohyphae form, and it is one of the few Candida species with the ability to reproduce sexually. In this study, we showed that a dpp3Δ mutant, inactivated for a putative pyrophosphatase, is impaired in cell separation, pseudohyphal growth and mating. The defective phenotypes were not restored after the reconstruction of a wild-type DPP3 locus, reinforcing the hypothesis of the presence of an additional mutation that we suspected in our previous study. Genetic crosses and genome sequencing identified an additional mutation in MED15, encoding a subunit of the mediator complex that functions as a general transcriptional co-activator in Eukaryotes. We confirm

SUBMITTER: Sabra A 

PROVIDER: S-EPMC10053558 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature