Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues.

ABSTRACT: Changes in the micro-environment of fibrous connective tissue can lead to alterations in the phenotypes of tissue-resident cells, yet the underlying mechanisms are poorly understood. Here, by visualizing the dynamics of histone spatial reorganization in tenocytes and mesenchymal stromal cells from fibrous tissue of human donors via super-resolution microscopy, we show that physiological and pathological chemomechanical cues can directly regulate the spatial nanoscale organization and density of chromatin in these tissue-resident cell populations. Specifically, changes in substrate stiffness, altered oxygen tension and the presence of inflammatory signals drive chromatin relocalization and compaction into the nuclear boundary, mediated by the activity of the histone methyltransferase EZH2 a

SUBMITTER: Heo SJ 

PROVIDER: S-EPMC10053755 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature