Fast-track transformation and genome editing in Brachypodium distachyon.

ABSTRACT:

Background

Even for easy-to-transform species or genotypes, the creation of transgenic or edited plant lines remains a significant bottleneck. Thus, any technical advance that accelerates the regeneration and transformation process is welcome. So far, methods to produce Brachypodium distachyon (Bd) transgenics span at least 14 weeks from the start of tissue culture to the recovery of regenerated plantlets.

Results

We have previously shown that embryogenic somatic tissues grow in the scutellum of immature zygotic Bd embryos within 3 days of in vitro induction with exogenous auxin and that the development of secondary embryos can be initiated immediately thereafter. Here, we further demonstrate that such pluripotent reactive tissues can be genetically transformed with Agrobact

SUBMITTER: Soulhat C 

PROVIDER: S-EPMC10053978 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature