Citrus Bright Spot Virus: A New Dichorhavirus, Transmitted by <i>Brevipalpus azores</i>, Causing Citrus Leprosis Disease in Brazil.

ABSTRACT: Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(-)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98-99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for ne

SUBMITTER: Chabi-Jesus C 

PROVIDER: S-EPMC10053991 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature