Bovine Enteroids as an In Vitro Model for Infection with Bovine Coronavirus.

ABSTRACT: Bovine coronavirus (BCoV) is one of the major viral pathogens of cattle, responsible for economic losses and causing a substantial impact on animal welfare. Several in vitro 2D models have been used to investigate BCoV infection and its pathogenesis. However, 3D enteroids are likely to be a better model with which to investigate host-pathogen interactions. This study established bovine enteroids as an in vitro replication system for BCoV, and we compared the expression of selected genes during the BCoV infection of the enteroids with the expression previously described in HCT-8 cells. The enteroids were successfully established from bovine ileum and permissive to BCoV, as shown by a seven-fold increase in viral RNA after 72 h. Immunostaining of differentiation markers showed a mixed popula

SUBMITTER: Shakya R 

PROVIDER: S-EPMC10054012 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature