Designing and fabrication of electrochemical nano-biosensor for the fast detection of SARS-CoV-2-RNA.

ABSTRACT: SARS-CoV-2 caused a global panic among populations. Rapid diagnostic procedures for the virus are crucial for disease control. Thus, the designed signature probe from a highly conserved region of the virus was chemically immobilized onto the nanostructured-AuNPs/WO₃-screen printed electrodes. Different concentrations of the matched oligonucleotides were spiked to test the specificity of the hybridization affinity whereas the electrochemical impedance spectroscopy was used for tracking the electrochemical performance. After a full assay optimization, limits of detection and quantification were calculated based on linear regression and were valued at 298 and 994 fM, respectively. Further, the high performance of the fabricated RNA-sensor chips was confirmed after testing the inter

SUBMITTER: Hussein HA 

PROVIDER: S-EPMC10054215 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature