Identification of m6A-associated LncRNAs as predict factors for the immune infiltration and prognosis of thyroid cancer.

ABSTRACT:

Objective

This study aims to evaluate the prognostic value of m6A-associated long noncoding RNAs (lncRNAs) and their interaction with tumour microenvironment in thyroid cancer (THCA).

Methods

The clinical and gene expression data of tumours from 502 patients with THCA and 58 adjacent normal tissues were retrieved from The Cancer Genome Atlas (TCGA)-THCA dataset. The Pearson test was utilized to identify potential m6A-associated lncRNAs (p < 0.001 and Pearson correlation coefficient > 0.4). Quantitative real-time polymerase chain reaction was performed to verify the expression levels of lncRNAs in tissues. MTT, EdU, colony formation and wound-healing assays were performed to determine the functions of m6A-associated lncRNAs in THCA cell proliferation and metastasis.

SUBMITTER: Su Y 

PROVIDER: S-EPMC10054316 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature