The Influence of Protein Charge and Molecular Weight on the Affinity of Aptamers.

ABSTRACT: Aptamers offer several advantages over antibodies. However, to ensure high affinity and specificity, a better understanding of the interactions between the nucleic-acid-based aptamers and their targets is mandatory. Therefore, we investigated the influence of two physical properties of proteins-molecular mass and charge-on the affinity of nucleic-acid-based aptamers. For this purpose, first, the affinity of two random oligonucleotides towards twelve proteins was determined. No binding was observed for proteins with a negative net charge towards the two oligonucleotides, while up to nanomolar affinity was determined for positively charged proteins with a high pI value. Second, a literature analysis comprising 369 aptamer-peptide/protein pairs was performed. The dataset included 296 differen

SUBMITTER: Drees A 

PROVIDER: S-EPMC10054347 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature