Metabolomics of bacterial-fungal pairwise interactions reveal conserved molecular mechanisms.

ABSTRACT: Bacterial-fungal interactions (BFIs) can shape the structure of microbial communities, but the small molecules mediating these BFIs are often understudied. We explored various optimization steps for our microbial culture and chemical extraction protocols for bacterial-fungal co-cultures, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that metabolomic profiles are mainly comprised of fungi derived features, indicating that fungi are the key contributors to small molecule mediated BFIs. LC-inductively coupled plasma MS (LC-ICP-MS) and MS/MS based dereplication using database searching revealed the presence of several known fungal specialized metabolites and structurally related analogues in these extracts, including siderophores such as desferrichrome, desferricoproge

SUBMITTER: Luu GT 

PROVIDER: S-EPMC10054941 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature