Cis-regulatory control of transcriptional timing and noise in response to estrogen.

ABSTRACT: Cis-regulatory elements control transcription levels, temporal dynamics, and cell-cell variation or transcriptional noise. However, the combination of regulatory features that control these different attributes is not fully understood. Here, we used single cell RNA-seq during an estrogen treatment time course and machine learning to identify predictors of expression timing and noise. We find that genes with multiple active enhancers exhibit faster temporal responses. We verified this finding by showing that manipulation of enhancer activity changes the temporal response of estrogen target genes. Analysis of transcriptional noise uncovered a relationship between promoter and enhancer activity, with active promoters associated with low noise and active enhancers linked to high noise. Finally

SUBMITTER: Ginley-Hidinger M 

PROVIDER: S-EPMC10054948 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature