A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis.

ABSTRACT: Human organoids have potential to revolutionize in vitro disease modeling by providing multicellular architecture and function that are similar to those in vivo . This innovative and evolving technology, however, still suffers from assay throughput and reproducibility to enable high-throughput screening (HTS) of compounds due to cumbersome organoid differentiation processes and difficulty in scale-up and quality control. Using organoids for HTS is further challenged by lack of easy-to-use fluidic systems that are compatible with relatively large organoids. Here, we overcome these challenges by engineering "microarray three-dimensional (3D) bioprinting" technology and associated pillar and perfusion plates for human organoid culture and analysis. High-precision, high-throughpu

SUBMITTER: Kang SY 

PROVIDER: S-EPMC10055006 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature