Growth-dependent gene expression variation influences the strength of codon usage biases.

ABSTRACT: The most highly expressed genes in microbial genomes tend to use a limited set of synonymous codons, often referred to as "preferred codons." The existence of preferred codons is commonly attributed to selection pressures on various aspects of protein translation including accuracy and/or speed. However, gene expression is condition-dependent and even within single-celled organisms transcript and protein abundances can vary depending on a variety of environmental and other factors. Here, we show that growth rate-dependent expression variation is an important constraint that significantly influences the evolution of gene sequences. Using large-scale transcriptomic and proteomic data sets in Escherichia coli and Saccharomyces cerevisiae, we confirm that codon usage biases are strongly associ

SUBMITTER: Johnson MM 

PROVIDER: S-EPMC10055066 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature