Universal recording of cell-cell contacts in vivo for interaction-based transcriptomics.

ABSTRACT: Cellular interactions are essential for tissue organization and functionality. In particular, immune cells rely on direct and usually transient interactions with other immune and non-immune populations to specify and regulate their function. To study these "kiss-and-run" interactions directly in vivo, we previously developed LIPSTIC (Labeling Immune Partnerships by SorTagging Intercellular Contacts), an approach that uses enzymatic transfer of a labeled substrate between the molecular partners CD40L and CD40 to label interacting cells. Reliance on this pathway limited the use of LIPSTIC to measuring interactions between CD4⁺ helper T cells and antigen presenting cells, however. Here, we report the development of a universal version of LIPSTIC (uLIPSTIC), which can record

SUBMITTER: Nakandakari-Higa S 

PROVIDER: S-EPMC10055214 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature