Efficient differential expression analysis of large-scale single cell transcriptomics data using dreamlet.

ABSTRACT: Advances in single-cell and -nucleus transcriptomics have enabled generation of increasingly large-scale datasets from hundreds of subjects and millions of cells. These studies promise to give unprecedented insight into the cell type specific biology of human disease. Yet performing differential expression analyses across subjects remains difficult due to challenges in statistical modeling of these complex studies and scaling analyses to large datasets. Our open-source R package dreamlet (DiseaseNeurogenomics.github.io/dreamlet) uses a pseudobulk approach based on precision-weighted linear mixed models to identify genes differentially expressed with traits across subjects for each cell cluster. Designed for data from large cohorts, dreamlet is substantially faster and uses less memory than

SUBMITTER: Hoffman GE 

PROVIDER: S-EPMC10055252 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature