Free ferrous ions sustain activity of mammalian stearoyl-CoA desaturase-1.

ABSTRACT: Mammalian stearoyl-CoA desaturase-1 (SCD1) introduces a double-bond to a saturated long-chain fatty acid and the reaction is catalyzed by a diiron center, which is well-coordinated by conserved histidine residues and is thought to remain with enzyme. However, we find that SCD1 progressively loses its activity during catalysis and becomes fully inactive after nine turnovers. Further studies show that the inactivation of SCD1 is due to the loss of an iron (Fe) ion in the diiron center, and that the addition of free ferrous ions (Fe ²⁺ ) sustains the enzymatic activity. Using SCD1 labeled with Fe isotope, we further show that free Fe ²⁺ is incorporated into the diiron center only during catalysis. We also discover that the diiron center in SCD1 has prominent electron par

SUBMITTER: Shen J 

PROVIDER: S-EPMC10055294 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature