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Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz.

ABSTRACT: Various implementations of mesoscopes provide optical access for calcium imaging across multi-millimeter fields-of-view (FOV) in the mammalian brain. However, capturing the activity of the neuronal population within such FOVs near-simultaneously and in a volumetric fashion has remained challenging since approaches for imaging scattering brain tissues typically are based on sequential acquisition. Here, we present a modular, mesoscale light field (MesoLF) imaging hardware and software solution that allows recording from thousands of neurons within volumes of 4000 × 200 μm, located at up to 400 μm depth in the mouse cortex, at 18 volumes per second. Our optical design and computational approach enable up to hour-long recording of ~10,000 neurons across multiple cortical areas in mice using workstation-grade computing resources.

SUBMITTER: Nobauer T

PROVIDER: S-EPMC10055306 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Mesoscale volumetric light field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz.

Nöbauer Tobias T Zhang Yuanlong Y Kim Hyewon H Vaziri Alipasha A

bioRxiv : the preprint server for biology 20230321

Various implementations of mesoscopes provide optical access for calcium imaging across multi-millimeter fields-of-view (FOV) in the mammalian brain. However, capturing the activity of the neuronal population within such FOVs near-simultaneously and in a volumetric fashion has remained challenging since approaches for imaging scattering brain tissues typically are based on sequential acquisition. Here, we present a modular, mesoscale light field (MesoLF) imaging hardware and software solution th ...[more]

PMID: 36993596

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