Continuous Multiplexed Phage Genome Editing Using Recombitrons.

ABSTRACT: Bacteriophages, which naturally shape bacterial communities, can be co-opted as a biological technology to help eliminate pathogenic bacteria from our bodies and food supply¹. Phage genome editing is a critical tool to engineer more effective phage technologies. However, editing phage genomes has traditionally been a low efficiency process that requires laborious screening, counter selection, or in vitro construction of modified genomes². These requirements impose limitations on the type and throughput of phage modifications, which in turn limit our knowledge and potential for innovation. Here, we present a scalable approach for engineering phage genomes using recombitrons: modified bacterial retrons³ that generate recombineering donor DNA paired wit

SUBMITTER: Fishman CB 

PROVIDER: S-EPMC10055335 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature