A simple method to dramatically increase C. elegans germline microinjection efficiency.

ABSTRACT: Genome manipulation methods in C. elegans require microinjecting DNA or ribonucleoprotein complexes into the microscopic core of the gonadal syncytium. These microinjections are technically demanding and represent a key bottleneck for all genome engineering and transgenic approaches in C. elegans . While there have been steady improvements in the ease and efficiency of genetic methods for C. elegans genome manipulation, there have not been comparable advances in the physical process of microinjection. Here, we report a simple and inexpensive method for handling worms using a paintbrush during the injection process that nearly tripled average microinjection rates compared to traditional worm handling methods. We found that the paintbrush increased injection throughput b

SUBMITTER: Gibney TV 

PROVIDER: S-EPMC10055348 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature