Comparing Vaginal and Endometrial Microbiota Using Culturomics: Proof of Concept.

ABSTRACT: It is generally accepted that microorganisms can colonize a non-pathological endometrium. However, in a clinical setting, endometrial samples are always collected by passing through the vaginal-cervical route. As such, the vaginal and cervical microbiomes can easily cross-contaminate endometrial samples, resulting in a biased representation of the endometrial microbiome. This makes it difficult to demonstrate that the endometrial microbiome is not merely a reflection of contamination originating from sampling. Therefore, we investigated to what extent the endometrial microbiome corresponds to that of the vagina, applying culturomics on paired vaginal and endometrial samples. Culturomics could give novel insights into the microbiome of the female genital tract, as it overcomes sequencing-re

SUBMITTER: Vanstokstraeten R 

PROVIDER: S-EPMC10055768 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature