The ENA1 Na⁺-ATPase Gene Is Regulated by the SPS Sensing Pathway and the Stp1/Stp2 Transcription Factors.

ABSTRACT: The Saccharomyces cerevisiae ENA1 gene, encoding a Na⁺-ATPase, responds transcriptionally to the alkalinization of the medium by means of a network of signals that involves the Rim101, the Snf1 and PKA kinases, and the calcineurin/Crz1 pathways. We show here that the ENA1 promoter also contains a consensus sequence, located at nt -553/-544, for the Stp1/2 transcription factors, the downstream components of the amino acid sensing SPS pathway. Mutation of this sequence or deletion of either STP1 or STP2 decreases the activity of a reporter containing this region in response to alkalinization as well as to changes in the amino acid composition in the medium. Expression driven from the entire ENA1 promoter was affected with similar potency by the d

SUBMITTER: Zekhnini A 

PROVIDER: S-EPMC10055992 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature