Rapid Reverse Purification DNA Extraction Approaches to Identify Microbial Pathogens in Wastewater.

ABSTRACT: Wastewater monitoring became a promising solution in the early detection of outbreaks. Despite the achievements in the identification of pathogens in wastewater using real-time PCR, there is still a lack of reliable rapid nucleic acid extraction protocols. Therefore, in this study, samples were subjected to alkali, proteinase K and/or bead-beating followed by reverse purification magnetic beads-based separation. Wastewater samples spiked with S. aureus, E. coli and C. parvum were used as examples for Gram-positive and -negative bacteria and protozoa, respectively. All results were compared with a spin column technology as a reference method. Proteinase K with bead beating (vortexing with 0.1 mm glass beads for three minutes) was particularly successful for bacterial DN

SUBMITTER: Schurig S 

PROVIDER: S-EPMC10056086 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature