Distinct Effects of Chemical Toxicity and Radioactivity on Metabolic Heat of Cultured Cells Revealed by "Isotope-Editing".

ABSTRACT: Studying the toxicity of chemical compounds using isothermal microcalorimetry (IMC), which monitors the metabolic heat from living microorganisms, is a rapidly expanding field. The unprecedented sensitivity of IMC is particularly attractive for studies at low levels of stressors, where lethality-based data are inadequate. We have revealed via IMC the effect of low dose rates from radioactive β^--decay on bacterial metabolism. The low dose rate regime (<400 µGyh^-1) is typical of radioactively contaminated environmental sites, where chemical toxicity and radioactivity-mediated effects coexist without a predominance or specific characteristic of either of them. We found that IMC allows distinguishing the two sources of metabolic interference on the basis of "isotope-editi

SUBMITTER: Oertel J 

PROVIDER: S-EPMC10056173 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature