Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications.

ABSTRACT: The Bacillus amyloliquefaciens RT7 strain was isolated from an extreme acidic environment and identified. The biodegradation capabilities of the strain using different carbon sources (glucose, oleic acid, Tween 80, PEG 200, and the combination of glucose-Tween 80) were evaluated via an indirect impedance technique. The glucose-Tween 80 combination was further studied using nuclear magnetic resonance (NMR). The exopolysaccharide (EPS_RT7) that had been produced with the strain when biodegrading glucose-Tween 80 was isolated and characterised using different techniques (GC-MS, HPLC/MSMS, ATR-FTIR, TGA, and DSC), and its molecular weight was estimated. The results show that the average molecular weight of EPS_RT7 was approximately 7.0794 × 10⁴ Da and a he

SUBMITTER: Sanchez-Leon E 

PROVIDER: S-EPMC10056187 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature