Investigating Circular RNAs Using qRT-PCR; Roundup of Optimization and Processing Steps.

ABSTRACT: Circular RNAs (circRNAs) have gained recent attraction due to their functional versatility and particular structure connected to human diseases. Current investigations are focused on the interplay between their ability to sponge smaller species of RNAs, such as microRNAs (miRNAs), thus influencing their regulatory activity on gene expression and protein templates. Therefore, their reported implication in various biological processes axis has resulted in an accumulating number of studies. While the testing and annotation methods of novel circular transcripts are still under development, there is still a plethora of transcript candidates suitable for investigation in human disease. The discordance in the literature regarding the approaches used in circRNAs quantification and validation metho

SUBMITTER: Drula R 

PROVIDER: S-EPMC10056686 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature