Generation of a Triple-Shuttling Vector and the Application in Plant Plus-Strand RNA Virus Infectious cDNA Clone Construction.

ABSTRACT: Infectious cloning of plant viruses is a powerful tool for studying the reverse genetic manipulation of viral genes in virus-host plant interactions, contributing to a deeper understanding of the life history and pathogenesis of viruses. Yet, most of the infectious clones of RNA virus constructed in E. coli are unstable and toxic. Therefore, we modified the binary vector pCass4-Rz and constructed the ternary shuttle vector pCA4Y. The pCA4Y vector has a higher copy number in the E. coli than the conventional pCB301 vector, can obtain a high concentration of plasmid, and is economical and practical, so it is suitable for the construction of plant virus infectious clones in basic laboratories. The constructed vector can be directly extracted from yeast and transformed into Ag

SUBMITTER: Feng C 

PROVIDER: S-EPMC10056883 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature